available restriction enzymes:
Forward Primer:
Reverse Primer:
pET-28a is a commonly used gene expression vector for protein synthesis in E. coli.it is a roughly 5300 bp plasmid that includes a T7 promoter, a modified lac operon, a KanR sequence (which confers kanamycin resistance) and many restriction enzyme sites. Within the MCS or multiple cloning site are sites for XhoI (CTCGAG), EagI (CGGCCG), HindIII (AAGCTT), SalI (GTCGAC), Eco53kI (GAGCTC), SacI (GAGCTC), EcoRI (GAATTC), & BamHI (GGATCC) enclosed by Histidine tags (which aid in purification). In order to obtain recombinant plasmids, both a forward and reverse promoter for the desired gene product must be prepared. The cDNA is then amplified using PCR, after which recombination can be facilitated using restriction enzymes. These catalysts must avoid acting on the gene of interest. The purpose of this page is to formulate both required promoters along with their respective enzymes.